Pro-tip for today: When attempting to create a website (I am using ASP.NET MVC 1.0) with IIS7 that allows anonymous users under an Active Directory domain it is imperative that you remember to give anonymous users read rights to the web directory on the system’s physical file system. Otherwise you could end up with pages that do not load the CSS correctly.

Furthermore, if you are running into assembly load problems when running on Windows Server 2008 R2 remember that everything is running in X64 and that you need to enable 32-bit compatibility in your Application Pools. To do this select the App Pool and click “Advanced Settings” and set “Enable 32-Bit Applications” to true.